1OMH

Conjugative Relaxase TrwC in complex with OriT Dna. Metal-free structure.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	110
Detector technology	CCD
Collection date	2002-03-08
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795, 0.9798, 0.9796
Spacegroup name	P 61 2 2
Unit cell lengths	90.830, 90.830, 203.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.950
R-factor	0.2487
Rwork	0.246
R-free	0.27970
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.692
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.050	1.950
Rmerge	0.146
Number of reflections	64999
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	4 *	25% PEGMM 2000, 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	4.6	4 *	25% PEGMM 2000, 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 296K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	(NH4)2(SO4)
2	1	1	(Na)(C2H3O2)
3	1	1	PEG
4	1	2	(Na)(C2H3O2)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	PEG2000 MME	25 (%(w/v))
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	sodium acetate	0.1 (M)	pH4.6