1OLT

Coproporphyrinogen III oxidase (HemN) from Escherichia coli is a Radical SAM enzyme.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-15
Detector	MARRESEARCH
Spacegroup name	P 63
Unit cell lengths	114.036, 114.036, 76.459
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.070
R-factor	0.155
Rwork	0.154
R-free	0.18700
Structure solution method	MAD
RMSD bond length	0.024 *
RMSD bond angle	2.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	autoSHARP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.110
High resolution limit [Å]	2.070	2.070
Rmerge	0.066	0.182
Number of reflections	34220 *
<I/σ(I)>	30.6	10.2
Completeness [%]	99.1	96.8
Redundancy	9.4	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	20 *	HANGING DROP: 5 MICROL PROTEIN + 5 MICROL RESEVOIR, PROTEIN: 10 MG/ML IN 10 MM HEPES PH 7.5, 3 MM DTT, 150 MM NACL, 0.01% (V/V) C8E4 RESEVOIR: 22% (W/V) PEG10000, 100 MM HEPES PH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	SAM	1 (mM)
3	1	reservoir	PEG10000	22 (%)
4	1	reservoir	HEPES	100 (mM)	pH7.0