1OLO

Hexameric Replicative DNA Helicase RepA from Plasmid RSF1010 - Cubic Crystal Structure

Experimental procedure

Experimental method	MAD
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-12-15
Detector	MARRESEARCH
Spacegroup name	P 43 3 2
Unit cell lengths	190.293, 190.293, 190.293
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.550
Rwork	0.176
R-free	0.21100
Structure solution method	MAD
Starting model (for MR)	1g8y
RMSD bond length	0.021 *
RMSD bond angle	1.838 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	150.000	2.580
High resolution limit [Å]	2.520	2.520
Rmerge	0.106	0.620 *
Total number of observations	305969 *
Number of reflections	40046
<I/σ(I)>	13.6	3.3
Completeness [%]	99.1	89.7
Redundancy	7.64	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	291	SITTING DROP VAPOR DIFFUSION AT 291K. PROTEIN STOCK: 26MG/ML REPA, 10MM TRIS-HCL PH8.0, 150MM NACL, 0.1MM EDTA. PRECIPITANT: 100MM TRIS-HCL PH 7.5, 26% PEG400, 100MM MGSO4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	26 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)	pH8.0
3	1	drop		150 (mM)
4	1	drop	EDTA	0.1 (mM)
5	1	reservoir	Tris-HCl	100 (mM)	pH7.5
6	1	reservoir	PEG400	26 (%)
7	1	reservoir		100 (mM)