1OKK

HOMO-HETERODIMERIC COMPLEX OF THE SRP GTPASES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-20
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	98.905, 98.905, 130.745
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 2.050
R-factor	0.156
Rwork	0.154
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ng1
RMSD bond length	0.010
RMSD bond angle	1.475 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BEAST
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.065	0.158
Number of reflections	41332
<I/σ(I)>	33.4	16.2
Completeness [%]	99.9	100
Redundancy	10.9	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	6.7		1.91 M AMMONIUM SULFATE, 0.1 M BISTRIS PH 6.7, 0.1 M NACL, 4% PEG 400.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1		protein	2000 (nM)
2	1		HEPES	50 (mM)	pH7.5