1OKJ
crystal structure of the essential E. coli YeaZ protein by MAD method using the gadolinium complex "DOTMA"
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Collection date | 2003-06-15 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.340, 97.600, 141.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.280 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.25700 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.850 | 2.360 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.045 | 0.136 |
Number of reflections | 48235 | |
<I/σ(I)> | 11 | 1.9 |
Completeness [%] | 98.3 | 98.5 |
Redundancy | 5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.7 | PEG 8000, NA ACETATE 0.1M PH 4.7, NACL 0.2M, GLYCEROL 10% |