1OK9

Decay accelerating factor (CD55): The structure of an intact human complement regulator.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-18
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	46.130, 54.600, 62.770
Unit cell angles	87.19, 85.61, 65.74

Refinement procedure

Resolution	20.000 - 3.000
R-free	0.27400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ojv
RMSD bond length	0.004
RMSD bond angle	21.300 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	TNT (5F)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	3.160
High resolution limit [Å]	3.000	3.000
Rmerge	0.053	0.114
Number of reflections	10372
<I/σ(I)>	9.1	5.8
Completeness [%]	93.0	92.7
Redundancy	3.8	3.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	4 *	0.2 M AMMONIUM SULPHATE, 20% M-PEG 5K, 0.1M SODIUM ACETATE PH 4.6, 16% GLYCEROL THEN SOAKED IN 2 MM K2PTCL4 FOR 6 DAYS

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.2 (M)
2	1	reservoir	MPEG5000	20 (%(w/v))
3	1	reservoir	sodium acetate	0.1 (M)	pH4.6
4	1	reservoir	glycerol	10 (%(w/v))