1OK3

Decay accelerating factor (cd55): the structure of an intact human complement regulator.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-15
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	46.670, 55.080, 61.650
Unit cell angles	86.47, 84.37, 64.91

Refinement procedure

Resolution	25.000 - 2.200
R-free	0.25300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h03
RMSD bond length	0.007
RMSD bond angle	19.590 *
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	TNT (5F)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.000 *	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.096	0.363 *
Number of reflections	26342
<I/σ(I)>	6.3	1.9
Completeness [%]	93.5	93.1
Redundancy	7.4	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	4 *	0.2 M AMMONIUM SULPHATE, 20% M-PEG 5K, 0.1M SODIUM ACETATE PH 4.6, 16% GLYCEROL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.2 (M)
2	1	reservoir	MPEG5000	20 (%(w/v))
3	1	reservoir	sodium acetate	0.1 (M)	pH4.6
4	1	reservoir	glycerol	10 (%(w/v))