1OJM
SPECIFICITY AND MECHANISM OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE: COMPLEX WITH UNSULPHATED CHONDROITIN DISACCHARIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-15 |
| Detector | OXFORD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.656, 104.266, 98.786 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.380 - 1.780 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c82 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.380 | 1.840 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.072 | 0.154 |
| Number of reflections | 70193 | |
| <I/σ(I)> | 17.1 | 4.6 |
| Completeness [%] | 84.2 | 76.5 |
| Redundancy | 2.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 3.5M AMMONIUM SULFATE, 200MM SODIUM CACODYLATE, PH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.4 |
| 3 | 1 | drop | EDTA | 150 (mM) | |
| 4 | 1 | drop | EDTA | 2 (mM) | |
| 5 | 1 | reservoir | chondroitin solution | 50 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 10 (mM) |
