1OJ7

STRUCTURAL GENOMICS, UNKNOWN FUNCTION CRYSTAL STRUCTURE OF E. COLI K-12 YQHD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-15
Detector	ADSC CCD
Spacegroup name	P 62
Unit cell lengths	237.925, 237.925, 66.744
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.000 - 2.000
R-factor	0.146
Rwork	0.145
R-free	0.16400
Structure solution method	OTHER
RMSD bond length	0.008
RMSD bond angle	1.115
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.047	0.158
Number of reflections	80905
<I/σ(I)>	9.3	4.6
Completeness [%]	95.3	92.7
Redundancy	2.5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0,95 M NA-CITRATE, 0.01 M NA-BORATE PH 8.5