1OJ5

Crystal structure of the Nco-A1 PAS-B domain bound to the STAT6 transactivation domain LXXLL motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS M18X
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-03-24
Detector	MARRESEARCH
Spacegroup name	P 62
Unit cell lengths	62.100, 62.100, 73.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.200 * - 2.210
R-factor	0.17
Rwork	0.166
R-free	0.21400
Structure solution method	OTHER
RMSD bond length	0.011
RMSD bond angle	1.394 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.200 *	2.250
High resolution limit [Å]	2.210	2.210
Rmerge	0.089	0.325
Total number of observations	137915 *
Number of reflections	8138
<I/σ(I)>	29.9	8.71
Completeness [%]	99.7	98.1
Redundancy	16.8	16.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	20 *	20 % PEG3350, 0.2 M LICL, pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	50 (mM)	pH7.0
2	1	drop		150 (mM)
3	1	drop	dithiothreitol	2 (mM)
4	1	drop	protein	19 (mg/ml)
5	1	reservoir		0.2 (M)
6	1	reservoir	PEG3350	20 (%)