1OIS

YEAST DNA TOPOISOMERASE I, N-TERMINAL FRAGMENT

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Detector technology	IMAGE PLATE
Collection date	1994-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	47.200, 53.070, 116.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.222
Rwork	0.222
R-free	0.29000
RMSD bond length	0.014
RMSD bond angle	1.730 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000 *
High resolution limit [Å]	1.850 *
Rmerge	0.061	0.278 *
Total number of observations	127777 *
Number of reflections	24425
Completeness [%]	95.1	80.1 *
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	20 (%)
2	1	reservoir	Tris-HCl	0.1 (M)