1OIL
STRUCTURE OF LIPASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 295 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1992-03-21 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.230, 47.420, 86.530 |
| Unit cell angles | 90.00, 116.11, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.25500 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.200 * |
| Data reduction software | FOURIER (SCALING) |
| Data scaling software | FOURIER (SCALING) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.040 * |
| Number of reflections | 29375 |
| Completeness [%] | 80.0 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.3 * | 22 * | Kim, K.K., (1992) J.Mol.Biol., 227, 1258. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 60-63 (%(v/v)) | |
| 2 | 1 | reservoir | sodium citrate | 200 (mM) | |
| 3 | 1 | reservoir | HEPES | 50 (mM) | |
| 4 | 1 | drop | MPD | 16 (%(v/v)) | |
| 5 | 1 | drop | sodium citrate | 100 (mM) | |
| 6 | 1 | drop | beta-octylglucoside | 0.36 (%(w/v)) | |
| 7 | 1 | drop | protein | 10 (mg/ml) | |
| 8 | 1 | drop | HEPES | 50 (mM) |
