1OID
5'-Nucleotidase (E. coli) with an Engineered Disulfide Bridge (S228C, P513C)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-03-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.496, 93.708, 82.901 |
Unit cell angles | 90.00, 97.71, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.169 |
Rwork | 0.166 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hp1 |
RMSD bond length | 0.029 |
RMSD bond angle | 2.170 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.055 | 0.320 |
Number of reflections | 194970 | |
<I/σ(I)> | 11.3 | 2.08 |
Completeness [%] | 98.4 | 90.8 |
Redundancy | 2.78 | 2.35 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 26% PEG 400, 100 MM CACODYLATE PH 6.5 |