1OI1
Crystal Structure of the MBT domains of Human SCML2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.762, 47.696, 41.493 |
| Unit cell angles | 90.00, 100.29, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.780 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| R-free | 0.20400 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE/RESOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.010 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.071 | 0.243 |
| Total number of observations | 117212 * | |
| Number of reflections | 21472 | 3022 * |
| <I/σ(I)> | 19.8 | 6.2 |
| Completeness [%] | 97.6 | 94.7 |
| Redundancy | 5.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | 4 * | 0.1 M TRIS, PH 8.0, 50MM MAGNESIUM CHLORIDE, 15.6% PEG4000 PEG200 (20%) - AS CRYOPROTECTANT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH7.5 |
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | reservoir | Tris | 100 (mM) | pH8.0 |
| 5 | 1 | reservoir | PEG4000 | 15.6 (%) | |
| 6 | 1 | reservoir | 50 (mM) |
