1OHU
Structure of Caenorhabditis elegans CED-9
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Temperature [K] | 100 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.790, 92.050, 47.640 |
Unit cell angles | 90.00, 99.78, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.030 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.24800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.089 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.030 | |
Rmerge | 0.042 | 0.201 * |
Number of reflections | 21636 | |
<I/σ(I)> | 17.7 | |
Completeness [%] | 84.1 | 54.2 * |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 285 * | 10% PEG 8K ,0.1M HEPES PH7.5, pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 10 (%) | |
2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
3 | 1 | drop | protein | 10 (mg/ml) | |
4 | 1 | drop | sodium phosphate | 20 (mM) | |
5 | 1 | drop | 50 (mM) | ||
6 | 1 | drop | beta-mercaptoethanol | 2 (mM) |