1OHL
YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE PUTATIVE CYCLIC REACTION INTERMEDIATE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-17 |
| Detector | ADSC CCD |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 103.200, 103.200, 167.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.700 - 1.600 |
| Rwork | 0.188 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ylv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.013 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | CCP4 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.055 | 0.388 |
| Number of reflections | 57568 | |
| <I/σ(I)> | 21.7 | 2.5 |
| Completeness [%] | 96.2 | 96.2 |
| Redundancy | 7.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | AS FOR PDB ENTRY 1AW5, pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ALA | 0.2 (mM) | |
| 2 | 1 | drop | enzyme | 2 (mg/ml) | |
| 3 | 1 | reservoir | PEG6000 | 2-10 (%(w/v)) | |
| 4 | 1 | reservoir | Tris-HCl | 200 (mM) | pH7.0-8.0 |
