1OHJ

HUMAN DIHYDROFOLATE REDUCTASE, MONOCLINIC (P21) CRYSTAL FORM

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	65.822, 40.350, 75.150
Unit cell angles	90.00, 109.74, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.208 *
Rwork	0.208
RMSD bond length	0.014
RMSD bond angle	0.052
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.500
Number of reflections	5276
Completeness [%]	93.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG4000	40 (%)
2	1	drop	sodium acetate	0.2 (M)
3	1	drop	Tris-HCl	0.1 (M)
4	1	reservoir	PEG4000	40 (%)
5	1	reservoir	sodium acetate	0.2 (M)
6	1	reservoir	Tris-HCl	0.1 (M)