Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 579.700, 626.650, 787.200 |
| Unit cell angles | 90.00, 89.90, 90.00 |
Refinement procedure
| Resolution | 190.130 - 3.450 |
| R-factor | 0.373 |
| Rwork | 0.373 |
| R-free | 0.36300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CRYO EM RECONSTRUCTION |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 190.000 |
| High resolution limit [Å] | 3.440 |
| Rmerge | 0.160 |
| Number of reflections | 4797606 |
| <I/σ(I)> | 9.6 |
| Completeness [%] | 63.0 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | Wikoff, W.R., (1998) Virology, 243, 113. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | potassium phosphate | 50 (mM) | pH7.0 |
| 2 | 1 | reservoir | PEG8000 | 1.0-1.5 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.9 (M) | |
| 4 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | drop | 2-mercaptoethanol | 1 (mM) | |
| 7 | 1 | drop | protein | 27-30 (mg/ml) |
