1OH4
Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate-binding module
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.790, 48.919, 96.421 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.220 - 1.350 |
| R-factor | 0.14 |
| Rwork | 0.138 |
| R-free | 0.17000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1of3 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.853 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.420 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.057 | 0.261 |
| Number of reflections | 31455 | |
| <I/σ(I)> | 17.5 | 2.9 |
| Completeness [%] | 97.8 | 87.3 |
| Redundancy | 4.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 1.5 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE PH 4.6 |
