1OGY
Crystal structure of the heterodimeric nitrate reductase from Rhodobacter sphaeroides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 123.000, 225.200, 154.600 |
Unit cell angles | 90.00, 92.10, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.200 |
R-factor | 0.2504 |
Rwork | 0.250 |
R-free | 0.26900 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2NAP 1jni |
RMSD bond length | 0.013 |
RMSD bond angle | 1.860 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.111 | 0.411 |
Number of reflections | 134043 | |
<I/σ(I)> | 6.3 | 1.8 |
Completeness [%] | 96.1 * | 96.1 |
Redundancy | 2 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.2 | VAPOUR-DIFFUSION. MIXING EQUAL AMOUNTS OF PROTEIN 25MG/ML., pH 8.20 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | bis-Tris-propane | 100 (%) | pH8.2 |
2 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
3 | 1 | reservoir | PEG4000 | 15 (%(w/v)) | |
4 | 1 | reservoir | 15 (mM) | ||
5 | 1 | drop | protein | 25 (mg/ml) |