1OGW

Synthetic Ubiquitin with fluoro-Leu at 50 and 67

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1999-05-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	50.840, 42.770, 28.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.320
R-factor	0.222
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubi
RMSD bond length	0.020
RMSD bond angle	0.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	6.000	1.320
High resolution limit [Å]	1.320	1.310
Rmerge	0.110	0.540
Total number of observations	157103 *
Number of reflections	14305 *
<I/σ(I)>	10	2.3
Completeness [%]	89.9	81.4
Redundancy	4.5	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	5.6		Love, S.G., (1997) Biochem. J., 323, 727. *