1OGP

The crystal structure of plant sulfite oxidase provides insight into sulfite oxidation in plants and animals

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-15
Detector	ADSC CCD
Spacegroup name	C 2 2 2
Unit cell lengths	222.918, 351.271, 158.282
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.600
R-factor	0.237
Rwork	0.235
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sox
RMSD bond length	0.034
RMSD bond angle	2.614
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.600 *	2.550
Rmerge	0.110	0.730 *
Total number of observations	1177360 *
Number of reflections	199819
<I/σ(I)>	21.6	2.03
Completeness [%]	99.5	99.5
Redundancy	5.9	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	9.2	18 *	5-12% JEFFAMINE 600, 50 MM TRIS/HCL, 10 MM CSCL, PH 9.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-60 (mg/ml)
2	1	reservoir	Jeffamine600	5-12 (%)
3	1	reservoir	Tris-HCl	50 (mM)
4	1	reservoir	CsCl	10 (mM)	pH9.2