1OGP
The crystal structure of plant sulfite oxidase provides insight into sulfite oxidation in plants and animals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 222.918, 351.271, 158.282 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.600 |
| R-factor | 0.237 |
| Rwork | 0.235 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sox |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.614 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.600 * | 2.550 |
| Rmerge | 0.110 | 0.730 * |
| Total number of observations | 1177360 * | |
| Number of reflections | 199819 | |
| <I/σ(I)> | 21.6 | 2.03 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 5.9 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 9.2 | 18 * | 5-12% JEFFAMINE 600, 50 MM TRIS/HCL, 10 MM CSCL, PH 9.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-60 (mg/ml) | |
| 2 | 1 | reservoir | Jeffamine600 | 5-12 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 4 | 1 | reservoir | CsCl | 10 (mM) | pH9.2 |
