1OGC

The Structure of Bacillus subtilis RbsD complexed with D-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	295
Spacegroup name	C 1 2 1
Unit cell lengths	123.654, 108.140, 83.357
Unit cell angles	90.00, 128.92, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.202
Rwork	0.202
R-free	0.23100
Structure solution method	DIRECT METHODS
RMSD bond length	0.005
RMSD bond angle	1.288
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000
Rmerge	0.049 *	0.249 *
Total number of observations	278205 *
Number of reflections	57573
Completeness [%]	94.3	85 *
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *		pH 6.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH7.4
3	1	drop		0.2 (M)
4	1	drop	dithiothreitol	2 (mM)
5	1	reservoir	PEG4000	20 (%)
6	1	reservoir	isopropyl	19 (%)
7	1	reservoir	sodium cacodylate	100 (mM)	pH6.5