1OFL
CRYSTAL STRUCTURE OF CHONDROITINASE B COMPLEXED TO DERMATAN SULFATE HEXASACCHARIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.574, 73.989, 59.063 |
| Unit cell angles | 90.00, 93.93, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.700 |
| R-factor | 0.135 |
| Rwork | 0.133 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dbg |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.839 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.08) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.980 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.041 | 0.149 |
| Number of reflections | 44254 | |
| <I/σ(I)> | 16.5 | 5.5 |
| Completeness [%] | 92.5 | 65.3 |
| Redundancy | 2.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.7 | PEG8000, 2-METHYL-2,4- PENTADIOL, AMMONIUM ACETATE, TRIS, pH 8.70 |
