1OFG

GLUCOSE-FRUCTOSE OXIDOREDUCTASE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-04
Detector	RIGAKU
Spacegroup name	P 21 21 2
Unit cell lengths	84.490, 283.690, 116.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.203 *
Rwork	0.203
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	22.900 *
Data reduction software	DENZO
Data scaling software	CCP4
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.700	2.700
Rmerge	0.090	0.265
Total number of observations	353200 *
Number of reflections	71500	7701 *
Completeness [%]	91.0	60
Redundancy	4.8	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		pH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG6000	5-15 (%(w/v))
3	1	reservoir	succinic acid/KOH	0.2 (M)	or 0.2 M citric acid/KOH