1OFE
Glutamate Synthase from Synechocystis sp in complex with 2-Oxoglutarate and L-DON at 2.45 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 122.669, 134.377, 198.774 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.450 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1llw |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.790 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.800 * | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.113 | 0.306 |
| Total number of observations | 1066424 * | |
| Number of reflections | 120397 | |
| Completeness [%] | 99.5 * | 99.9 * |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 4 * | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | drop | HEPES-KOH | 20 (mM) | |
| 3 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 4 | 1 | drop | 2-OG | 2 (mM) | |
| 5 | 1 | drop | dithiothreitol | 5 (mM) | pH7.5 |
| 6 | 1 | reservoir | PEG4000 | 20-26 (%(w/v)) | |
| 7 | 1 | reservoir | Tris-HCl | 100 | |
| 8 | 1 | reservoir | sodium acetate | 200 (mM) | pH8.0 |
