1OFE

Glutamate Synthase from Synechocystis sp in complex with 2-Oxoglutarate and L-DON at 2.45 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	122.669, 134.377, 198.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.450
R-factor	0.209
Rwork	0.207
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1llw
RMSD bond length	0.017
RMSD bond angle	1.790 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	111.800 *	2.580
High resolution limit [Å]	2.450	2.450
Rmerge	0.113	0.306
Total number of observations	1066424 *
Number of reflections	120397
Completeness [%]	99.5 *	99.9 *
Redundancy	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	4 *	pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	18 (mg/ml)
2	1	drop	HEPES-KOH	20 (mM)
3	1	drop	glycerol	10 (%(v/v))
4	1	drop	2-OG	2 (mM)
5	1	drop	dithiothreitol	5 (mM)	pH7.5
6	1	reservoir	PEG4000	20-26 (%(w/v))
7	1	reservoir	Tris-HCl	100
8	1	reservoir	sodium acetate	200 (mM)	pH8.0