1OFB
CRYSTAL STRUCTURE OF THE TYROSINE-REGULATED 3-DEOXY-D-ARABINO-HEPTULOSONATE-7-PHOSPHATE SYNTHASE FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH MANGANESE(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.636, 50.502, 64.930 |
| Unit cell angles | 90.00, 106.36, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.010 |
| Rwork | 0.161 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hfb |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.932 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.032 | 0.191 |
| Number of reflections | 76999 | |
| <I/σ(I)> | 15.98 | 3.82 |
| Completeness [%] | 97.4 | 82.7 |
| Redundancy | 1.87 | 1.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | TRIS PH 7.5-9.0 10 MM, 20% PEG3400, 5% GLYCEROL, 2.4 EQUIV. MN, 13-17MG/ML DAHPS |
