1OF8

double complex of the tyrosine sensitive DAHP Synthase from S. cerevisiae with Co2+, PEP and the E4P analogoue G3P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-07
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	196.462, 50.788, 64.700
Unit cell angles	90.00, 106.39, 90.00

Refinement procedure

Resolution	27.000 - 1.500
R-factor	0.1266
Rwork	0.124
R-free	0.16710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MOLECULE A OF PDB ENTRY 1HFB
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.300	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.057	0.320
Number of reflections	234883
<I/σ(I)>	9.75	2.82
Completeness [%]	96.6	96.3
Redundancy	2.4	1.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		pH 8.50