1OEY
Heterodimer of p40phox and p67phox PB1 domains from human NADPH oxidase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-15 |
Detector | ADSC CCD |
Wavelength(s) | 0.97926,0.97912,0.9757959 |
Spacegroup name | P 64 |
Unit cell lengths | 151.422, 151.422, 68.317 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 129.000 * - 2.000 |
R-factor | 0.212 |
Rwork | 0.210 |
R-free | 0.25000 * |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.400 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.155 | 0.473 |
Total number of observations | 357868 * | |
Number of reflections | 59062 * | |
<I/σ(I)> | 8.6 | 1.6 |
Completeness [%] | 97.7 | 94 |
Redundancy | 6.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 9 | 17 * | 18% PEG 3350, 17% PEG 400, 4.8% ISOPROPYL ALCOHOL, 0.1 M CAPSO PH 9.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.5 (mg/ml) | |
10 | 1 | reservoir | PEG400 | 17 (%) | |
11 | 1 | reservoir | CAPSO | 0.1 (M) | pH9.0 |
2 | 1 | drop | Tris | 20 (mM) | pH7.4 |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | drop | PEG3350 | 18 (%) | |
6 | 1 | drop | PEG400 | 17 (%) | |
7 | 1 | drop | isopropanol | 4.8 (%) | |
8 | 1 | drop | CAPSO | 0.1 (M) | pH9.0 |
9 | 1 | reservoir | PEG3350 | 18 (%) |