1OEK
YodA from Escherichia coli crystallised with zinc ions
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.953 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.600, 58.600, 152.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.050 - 2.400 |
| R-factor | 0.273 |
| Rwork | 0.269 |
| R-free | 0.35000 * |
| Structure solution method | MAD |
| RMSD bond length | 0.018 * |
| RMSD bond angle | 2.278 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.050 | 2.270 |
| High resolution limit [Å] | 2.400 * | 2.200 |
| Rmerge | 0.086 | 0.350 * |
| Number of reflections | 10445 * | |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 98.6 | 71.1 * |
| Redundancy | 12. * | 9.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 6.5 | David, G., (2002) Acta Cryst., D58, 1243. * |
