1OE5

Xenopus SMUG1, an anti-mutator uracil-DNA Glycosylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-15
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	120.596, 85.653, 78.467
Unit cell angles	90.00, 118.56, 90.00

Refinement procedure

Resolution	42.600 * - 2.300
R-factor	0.166
Rwork	0.163
R-free	0.22000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NATIVE STRUCTURE
RMSD bond length	0.031
RMSD bond angle	2.568 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.600 *	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.066	0.222
Number of reflections	29920
<I/σ(I)>	6.6	3
Completeness [%]	95.8	77.1
Redundancy	4.0 *	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	20 *	pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	18-25 (%(w/v))
2	1	reservoir	isopropanol	2-12 (%(v/v))
3	1	reservoir	HEPES-NaOH	100 (mM)	pH7 or 8
4	1	drop	protein	0.5-7 (mg/ml)