1OE4

Xenopus SMUG1, an anti-mutator uracil-DNA Glycosylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-15
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	121.681, 85.936, 79.126
Unit cell angles	90.00, 118.36, 90.00

Refinement procedure

Resolution	69.000 * - 2.000
R-factor	0.179
Rwork	0.177
R-free	0.19600 *
Structure solution method	MIRAS
RMSD bond length	0.024 *
RMSD bond angle	2.081 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE/RESOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.000 *	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.113 *	0.289 *
Number of reflections	48502 *
<I/σ(I)>	4.7	1.3
Completeness [%]	100.0 *	100
Redundancy	5.4 *	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	20 *	pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	18-25 (%(w/v))
2	1	reservoir	isopropanol	2-12 (%(v/v))
3	1	reservoir	HEPES-NaOH	100 (mM)	pH7 or 8
4	1	drop	protein	0.5-7 (mg/ml)