1OE0

CRYSTAL STRUCTURE OF DROSOPHILA DEOXYRIBONUCLEOSIDE KINASE IN COMPLEX WITH DTTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-18
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	67.078, 119.680, 69.512
Unit cell angles	90.00, 92.85, 90.00

Refinement procedure

Resolution	24.730 - 2.400
R-factor	0.22
Rwork	0.220
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j90
RMSD bond length	0.011
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.062	0.230
Number of reflections	42341
<I/σ(I)>	22	4.2
Completeness [%]	99.9	98.9
Redundancy	5.5	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.50