1OD3
Structure of CSCBM6-3 From Clostridium stercorarium in complex with laminaribiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.183, 52.154, 64.755 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 * - 1.000 |
R-factor | 0.132 |
Rwork | 0.131 |
R-free | 0.14600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nae |
RMSD bond length | 0.018 |
RMSD bond angle | 2.003 * |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.060 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.053 | 0.409 |
Number of reflections | 61302 | |
<I/σ(I)> | 17.8 | 3.3 |
Completeness [%] | 97.2 | 94.2 |
Redundancy | 4.5 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.60 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 17 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 26 (%(w/v)) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
4 | 1 | reservoir | ammonium sulfate | 0.2 (M) |