1OBF

The crystal structure of Glyceraldehyde 3-phosphate Dehydrogenase from Alcaligenes xylosoxidans at 1.7A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-15
Detector	ADSC CCD
Spacegroup name	C 2 2 21
Unit cell lengths	88.984, 146.622, 146.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.16701
Rwork	0.166
R-free	0.18900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gd1
RMSD bond length	0.012 *
RMSD bond angle	1.497 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.099	0.350 *
Number of reflections	104690 *
<I/σ(I)>	22	2.2
Completeness [%]	97.6	90.7
Redundancy	6. *	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		PROTEIN 5MG/ML, 12 % PEG550, 100 MM POTASSIUM THIOCYANATE, 50 MM MES BUFFER (PH 6.5)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	PEG550	12 (%)
3	1	drop	potassium thiocyanate	100 (mM)
4	1	drop	MES	50 (mM)	pH6.5
5	1	reservoir	PEG550	24.5 (%)
6	1	reservoir	potassium thiocyanate	200 (mM)
7	1	reservoir	MES	100 (mM)	pH6.5