1OBA
Multimodular Pneumococcal Cell Wall Endolysin from phage Cp-1 complexed with choline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.030, 95.950, 129.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.000 - 2.450 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h09 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.098 | 0.388 |
| Number of reflections | 17522 | |
| <I/σ(I)> | 7.2 | 2 |
| Completeness [%] | 96.1 | 96.1 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 1.7M NA FORMATE, 0.1M NA CITRATE PH 6.0,1.8MM N-DECYL-MALTOSIDE |
