1OB8
Holliday Junction Resolving Enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-04 |
Detector | ADSC CCD |
Spacegroup name | P 65 |
Unit cell lengths | 90.577, 90.577, 70.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.540 - 1.800 |
Rwork | 0.197 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SE-SAD DERIVED STRUCTURE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.292 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 30127 | |
<I/σ(I)> | 41.1 | 1.75 |
Completeness [%] | 98.0 | 94.1 |
Redundancy | 4.25 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | 293 | 0.2M LITHIUM SULFATE,0.1M TRIS PH 9 20% PEG 4000,15% ETHYLENE GLYCOL,293K |