1OB1
Crystal structure of a Fab complex whith Plasmodium falciparum MSP1-19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.750, 213.460, 59.890 |
| Unit cell angles | 90.00, 100.95, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.900 |
| R-factor | 0.258 |
| Rwork | 0.257 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2IGF 1a3r 1b9w |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.071 | 0.578 |
| Number of reflections | 27530 | |
| <I/σ(I)> | 16.5 | 2.6 |
| Completeness [%] | 96.8 | 87.6 |
| Redundancy | 3.34 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | 291 * | Pizarro, J.C., (2001) Acta Crystallogr.,Sect.D, D58, 1246. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH7.4 |
| 3 | 1 | reservoir | sodium acetate | 0.075 (M) | |
| 4 | 1 | reservoir | sodium cacodylate | 0.038 (M) | |
| 5 | 1 | reservoir | PEG8000 | 11.3 (%) | |
| 6 | 1 | reservoir | dioxane | 3 (%) | pH6.5 |
