1OAY

Antibody multispecificity mediated by conformational diversity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-11-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	79.174, 79.142, 168.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400 *
R-factor	0.226
Rwork	0.258
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ANQ
RMSD bond length	0.013 *
RMSD bond angle	1.500 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.268	2.810
High resolution limit [Å]	2.400 *	2.660
Rmerge	0.126 *	0.583 *
Number of reflections	47809 *
<I/σ(I)>	6.6	3.1
Completeness [%]	99.2 *	94.7 *
Redundancy	6.7 *	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *		0 22% PEG 8K 0.1 M SODIUM CACODYLATE, 0.2M SODIUM ACETATE, PH 5.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	22 (%)
2	1	reservoir	sodium cacodylate	0.1 (M)
3	1	reservoir	sodium acetate	0.2 (M)	pH5.0