1OAG
Ascorbate peroxidase from soybean cytosol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 81.842, 81.842, 75.031 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.740 - 1.750 |
| R-factor | 0.14375 |
| Rwork | 0.142 |
| R-free | 0.18200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1apx |
| RMSD bond length | 0.020 * |
| RMSD bond angle | 1.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.740 * | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.067 | 0.320 |
| Total number of observations | 92501 * | |
| Number of reflections | 26092 | |
| <I/σ(I)> | 16.1 | 2.4 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.3 | 2.25M LI2SO4, 0.1M HEPES, PH8.3, pH 8.30 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | lithium sulfate | 2.25 (M) | |
| 3 | 1 | reservoir | HEPES | 0.1 (M) | pH8.3 |
