1OAA
MOUSE SEPIAPTERIN REDUCTASE COMPLEXED WITH NADP AND OXALOACETATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 115.880, 115.880, 103.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 1.250 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.22200 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.581 |
| Data reduction software | MOSFLM (V. 5.23) |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.068 | |
| Total number of observations | 630228 * | |
| Number of reflections | 112800 | |
| Completeness [%] | 99.6 | 97.3 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.5 (mg/ml) | |
| 2 | 1 | drop | MES | 20 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | NADP | 10 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 6 | 1 | reservoir | citrate | 0.1 (M) |
