1OA9

Structure of Melanocarpus albomyces endoglucanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2001-07-15
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	47.304, 47.304, 177.328
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.000
R-factor	0.215
Rwork	0.215
R-free	0.27600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eng
RMSD bond length	0.006
RMSD bond angle	1.410 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.060
High resolution limit [Å]	2.000	2.000
Rmerge	0.079	0.290
Total number of observations	296601 *
Number of reflections	13270 *
<I/σ(I)>	8	1.5
Completeness [%]	91.2	87.4
Redundancy	22.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6		Hirvonen, M., (2002) Acta Cryst., D58, 336. *