1OA7

Structure of Melanocarpus albomyces endoglucanase in complex with cellobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-07-15
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	47.300, 47.300, 178.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.191
Rwork	0.191
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eng
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.060
High resolution limit [Å]	2.000	2.000
Rmerge	0.084 *	0.408
Total number of observations	133688 *
Number of reflections	13447 *
<I/σ(I)>	12.1	3.6
Completeness [%]	92.4	89.1
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6		Hirvonen, M., (2002) Acta Cryst., D58, 336. *