1O9G

rRNA methyltransferase aviRa from Streptomyces viridochromogenes at 1.5A

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Collection date	2002-09-08
Spacegroup name	P 1 21 1
Unit cell lengths	36.362, 47.320, 63.385
Unit cell angles	90.00, 99.44, 90.00

Refinement procedure

Resolution	25.000 * - 1.500
R-factor	0.17
Rwork	0.169
R-free	0.20200
Structure solution method	MAD
RMSD bond length	0.012 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *	1.520
High resolution limit [Å]	1.500	1.500
Rmerge	0.039 *	0.200 *
Total number of observations	289006 *
Number of reflections	64711	1482 *
<I/σ(I)>	38.7	6.6
Completeness [%]	97.0	90
Redundancy	4.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.9	20 *	PEG 20000 9%, MES 6.6, pH 6.90

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	reservoir	MES	100 (mM)	pH6.6-6.9
3	1	reservoir	PEG20000	6-8 (%(w/v))