1O87
A new MgGDP complex of the Ffh NG domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-06-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.415, 113.631, 61.382 |
| Unit cell angles | 90.00, 111.76, 90.00 |
Refinement procedure
| Resolution | 56.800 - 2.100 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.23600 * |
| Structure solution method | MAD |
| Starting model (for MR) | 1ffh |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.669 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.045 | 0.159 |
| Number of reflections | 40999 | |
| <I/σ(I)> | 24.1 | 6.1 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 4.0 M SODIUM FORMATE, pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 32 (mg/ml) | |
| 2 | 1 | drop | 2 (mM) | ||
| 3 | 1 | drop | GTP | 2 (mM) | |
| 4 | 1 | reservoir | sodium formate | 4 (M) |
