1O7Y

Crystal structure of IP-10 M-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-12-04
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	138.979, 53.722, 53.366
Unit cell angles	90.00, 105.72, 90.00

Refinement procedure

Resolution	20.000 * - 3.000
R-factor	0.267
Rwork	0.267
R-free	0.30900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rhp
RMSD bond length	0.009
RMSD bond angle	24.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.096	0.434
Total number of observations	57731 *
Number of reflections	7390
<I/σ(I)>	10.1	2.6
Completeness [%]	95.4	92.8
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.4	16 *	10MG/ML PROTEIN, 16% PEG 4000, 0.1M SODIUM ACETATE BUFFER, PH 4.4, 0.2M AMMONIUM SULPHATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)	in water
2	1	reservoir	sodium acetate	0.1 (M)	pH4.4
3	1	reservoir	PEG4000	16 (%)
4	1	reservoir	ammonium sulfate	0.2 (M)