1O7L
Molybdate-activated form of ModE from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 43 |
| Unit cell lengths | 78.831, 78.831, 195.099 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.750 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.25500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h9r |
| RMSD bond length | 0.004 |
| RMSD bond angle | 24.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.810 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.099 | 0.377 |
| Total number of observations | 124769 * | |
| Number of reflections | 2992 * | |
| <I/σ(I)> | 15.1 | 3.8 |
| Completeness [%] | 96.0 | 99.6 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 27% PEG1000, 70MM CACL2, 1.5% 1,2,3-HEPTANETRIOL, 70MM HEPES PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium HEPES | 70 (mM) | pH7.5 |
| 2 | 1 | reservoir | PEG1000 | 27 (%(w/v)) | |
| 3 | 1 | reservoir | 70 (mM) | ||
| 4 | 1 | drop | heptanetriol | 1.5 (%) | |
| 5 | 1 | drop | 2.2 (mM) | ||
| 6 | 1 | drop | protein | 3.9 (mg/ml) | |
| 7 | 1 | drop | DNA oligonucleotide | 0.140 (mM) |
