1O7I
Crystal structure of a single stranded DNA binding protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 130 |
Detector technology | CCD |
Collection date | 2002-03-15 |
Detector | ADSC CCD |
Wavelength(s) | 0.9600, 0.9780, 0.9786 |
Spacegroup name | P 61 |
Unit cell lengths | 75.805, 75.805, 70.118 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 65.000 - 1.260 * |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.21000 * |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.600 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.920 * | 1.730 |
High resolution limit [Å] | 1.260 * | 1.260 * |
Rmerge | 0.081 * | 0.352 |
Total number of observations | 463924 * | |
Number of reflections | 60733 * | |
<I/σ(I)> | 8.1 | 2.2 |
Completeness [%] | 99.9 * | 99.9 * |
Redundancy | 7.5 * | 3.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | Kerr, I.D., (2001) Acta Crystallogr.,Sect.D, 57, 1290. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2.4 (M) | |
3 | 1 | reservoir | citric acid | 100 (mM) | pH3.5-5.0 |