1O71
Crystal structure of the water-soluble state of the pore-forming cytolysin Sticholysin II complexed with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.860, 43.570, 120.030 |
| Unit cell angles | 90.00, 92.43, 90.00 |
Refinement procedure
| Resolution | 9.980 - 2.260 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.26900 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Refinement software | CNS (1.0) |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M TRIS, PH 7.5, 0.2 M LITHIUM SULFATE, 30%(W/V) PEG 4000 |
