1O5O
Crystal structure of Uracil phosphoribosyltransferase (TM0721) from Thermotoga maritima at 2.30 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-24 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.078, 87.406, 90.826 |
| Unit cell angles | 90.00, 115.29, 90.00 |
Refinement procedure
| Resolution | 30.930 - 2.300 |
| R-factor | 0.17004 |
| Rwork | 0.167 |
| R-free | 0.23635 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i5e |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.702 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 41796 | |
| <I/σ(I)> | 10.16 | 1.49 |
| Completeness [%] | 100.0 | 99.71 |
| Redundancy | 3.72 | 3.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 9 | 277 | 2.4M ammonium sulfate, 0.1M bicine pH 9.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
